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Caldesmon (C21): m-IgG Fc BP-HRP Bundle

SC-525673
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityAvian, Human, Mouse, Porcine, Rat
TargetCALD1
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Caldesmon (C21): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID800
  • Target name
    CALD1
  • Target description
    caldesmon 1
  • Target synonyms
    CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, h-CD, caldesmon, testis secretory sperm-binding protein Li 227n
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Avian, Human, Mouse, Porcine, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caldesmon using anti-Caldesmon antibody (PB9922). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caldesmon antigen affinity purified polyclonal antibody (Catalog # PB9922) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caldesmon at approximately 80 kDa. The expected band size for Caldesmon is at 93 kDa.
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