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ProductsBack/Caldesmon antibody [N1N2], N-term
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE GTX102498 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE GTX102498 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE GTX102498 diluted at 1:1000

Caldesmon antibody [N1N2], N-term

GTX102498
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCALD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caldesmon antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.86 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID800
  • Target name
    CALD1
  • Target description
    caldesmon 1
  • Target synonyms
    CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, h-CD, caldesmon, testis secretory sperm-binding protein Li 227n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05682
  • Protein Name
    Caldesmon
  • Scientific Description
    This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caldesmon using anti-Caldesmon antibody (PB9922). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caldesmon antigen affinity purified polyclonal antibody (Catalog # PB9922) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caldesmon at approximately 80 kDa. The expected band size for Caldesmon is at 93 kDa.
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