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Calnexin antibody [C3], C-term

GTX109669
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCANX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calnexin antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    calnexin; CNX; epididymis secretory sperm binding protein; IP90; major histocompatibility complex class I antigen-binding protein p88; P90
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27824
  • Protein Name
    Calnexin
  • Scientific Description
    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Outcome of severely injured patients in a unique trauma system with 24/7 double trauma surgeon on-call service.
    Read more
  • Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer.
    Read more
  • Elexacaftor/VX-445-mediated CFTR interactome remodeling reveals differential correction driven by mutation-specific translational dynamics.
    Read more
  • Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.
    Read more
  • Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma. Gao G et al., 2022, Am J Cancer Res
    Read more
  • Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation. Vultaggio-Poma V et al., 2022, Theranostics
    Read more
  • P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells. Pegoraro A et al., 2021 Nov 16, Cell Death Dis
    Read more
  • Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis. Wright MT et al., 2021, Mol Cell Proteomics
    Read more
  • NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma. Hu W et al., 2021 Feb 8, JCI Insight
    Read more
  • Comparative Multiplexed Interactomics of SARS-CoV-2 and Homologous Coronavirus Nonstructural Proteins Identifies Unique and Shared Host-Cell Dependencies. Davies JP et al., 2020 Dec 11, ACS Infect Dis
    Read more

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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