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Calnexin antibody [N3C2], Internal

GTX101676
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCANX
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calnexin antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.16 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    calnexin; CNX; epididymis secretory sperm binding protein; IP90; major histocompatibility complex class I antigen-binding protein p88; P90
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27824
  • Protein Name
    Calnexin
  • Scientific Description
    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Influence of short term storage conditions, concentration methodsand excipients on extracellular vesicle recovery and function. van de Wakker SI et al., 2022 Jan, Eur J Pharm Biopharm
    Read more
  • Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk. Wijenayake S et al., 2021, PLoS One
    Read more
  • A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. de Jong OG et al., 2020 Feb 28, Nat Commun
    Read more
  • Potential of mesenchymal- and cardiac progenitor cells for therapeutic targeting of B-cells and antibody responses in end-stage heart failure. van den Hoogen P et al., 2019, PLoS One
    Read more
  • Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine. Ilahibaks NF et al., 2019 Nov 25, Cells
    Read more
  • Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis. Costa Verdera H et al., 2017 Nov 28, J Control Release
    Read more
  • Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins. Sever L et al., 2014 Feb, Dev Comp Immunol
    Read more

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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