More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Calnexin antibody [N3C2], Internal
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [N3C2], Internal

GTX101676
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCANX
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Calnexin antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.16 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    CNX, IP90, P90, calnexin, epididymis secretory sperm binding protein, major histocompatibility complex class I antigen-binding protein p88
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27824
  • Protein Name
    Calnexin
  • Scientific Description
    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • van de Wakker SI, van Oudheusden J, Mol EA, et al. Influence of short term storage conditions, concentration methodsand excipients on extracellular vesicle recovery and function. Eur J Pharm Biopharm. 2022,170:59-69. doi: 10.1016/j.ejpb.2021.11.012
    Read this paper
  • Wijenayake S, Eisha S, Tawhidi Z, et al. Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk. PLoS One. 2021,16(9):e0257633. doi: 10.1371/journal.pone.0257633
    Read this paper
  • de Jong OG, Murphy DE, Mäger I, et al. A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. Nat Commun. 2020,11(1):1113. doi: 10.1038/s41467-020-14977-8
    Read this paper
  • van den Hoogen P, de Jager SCA, Mol EA, et al. Potential of mesenchymal- and cardiac progenitor cells for therapeutic targeting of B-cells and antibody responses in end-stage heart failure. PLoS One. 2019,14(12):e0227283. doi: 10.1371/journal.pone.0227283
    Read this paper
  • Ilahibaks NF, Lei Z, Mol EA, et al. Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine. Cells. 2019,8(12). doi: 10.3390/cells8121509
    Read this paper
  • Costa Verdera H, Gitz-Francois JJ, Schiffelers RM, et al. Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis. J Control Release. 2017,266:100-108. doi: 10.1016/j.jconrel.2017.09.019
    Read this paper
  • Sever L, Vo NT, Bols NC, et al. Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins. Dev Comp Immunol. 2014,42(2):211-9. doi: 10.1016/j.dci.2013.09.005
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-CANX Antibody144-63846
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
Product group Antibodies
Boster Bio
Anti-Calnexin/CANX Antibody Picoband(r)A03372-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCANX
  • SizePrice
Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
Calnexin antibody [C3], C-termGTX109669
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat, Sheep
TargetCANX
  • SizePrice
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
Calnexin antibodyGTX112886
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
FACS analysis of HeLa (upper) and A549 (lower) cells using GTX57717 Calnexin antibody. Cell Number: 1 x 10? cells Red: Primary antibody Antibody amount: 2-5 μg
Product group Antibodies
GeneTex
Calnexin antibody [AT18B9]GTX57717
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCANX
  • SizePrice
Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
References
GeneTex
Calnexin antibody [GT1563]GTX629976
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
Calnexin antibody [HL1598]GTX637077
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Feline, Human, Mouse, Rat
TargetCANX
  • SizePrice
Product group Antibodies
Biomatik
CANX Polyclonal AntibodyCAC14640
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCANX
  • SizePrice
Product group Antibodies
References
Bioss
Calnexin Polyclonal AntibodyBS-1693R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Chicken, Equine, Human, Mouse, Rabbit, Rat
TargetCANX
  • SizePrice
Western Blot analysis of COLO205 cells using Calnexin Polyclonal Antibody
Product group Antibodies
Cusabio
CANX AntibodyCSB-PA001175
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice