IHC-P analysis of human hepatocarcinoma tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human kidney tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_1129_IHC-P_w_23060620_301.webp "IHC-P analysis of human kidney tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![WB analysis of 25 ug of HepG2 lysate using GTX22798 Calnexin antibody [AF18]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_1598_WB_w_23060620_972.webp "WB analysis of 25 ug of HepG2 lysate using GTX22798 Calnexin antibody [AF18]. Dilution : 1:500")
![ICC/IF analysis of transfected COS7 cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Permeabilization : 0.1% Triton X-100 Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_2003_ICC-IF_w_23060620_787.webp "ICC/IF analysis of transfected COS7 cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Permeabilization : 0.1% Triton X-100 Dilution : 1:500")
![ICC/IF analysis of A549 cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_466_ICC-IF_w_23060620_862.webp "ICC/IF analysis of A549 cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei")
![ICC/IF analysis of HMVEC Cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_467_ICC-IF_w_23060620_841.webp "ICC/IF analysis of HMVEC Cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei")
![ICC/IF analysis of U-87 MG cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde Permeabilization : 0.05% Triton X-100 in PBS for 60 seconds at room temperature Dilution : 1:100 for 2 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22798/GTX22798_468_ICC-IF_w_23060620_361.webp "ICC/IF analysis of U-87 MG cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde Permeabilization : 0.05% Triton X-100 in PBS for 60 seconds at room temperature Dilution : 1:100 for 2 hours at room temperature")
IHC-P analysis of human hepatocarcinoma tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
Calnexin antibody [AF18]
GTX22798
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetCANX
Overview
- SupplierGeneTex
- Product NameCalnexin antibody [AF18]
- Delivery Days Customer9
- Antibody SpecificityImmunocytochemical staining of calnexin with GTX22798 in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining.
- Application Supplier NoteWB: 1:100-1:1000. ICC/IF: 1:50-1:500. IHC-Fr: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAF18
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID821
- Target nameCANX
- Target descriptioncalnexin
- Target synonymscalnexin; CNX; epididymis secretory sperm binding protein; IP90; major histocompatibility complex class I antigen-binding protein p88; P90
- HostMouse
- IsotypeIgG1
- Protein IDP27824
- Protein NameCalnexin
- Scientific DescriptionThis gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
- ReactivityHuman, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Comparison of five commercial extraction kits for subsequent membrane protein profiling. Buenger S et al., 2009 Dec, CytotechnologyRead more