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Calpain 3 antibody [C2C3], C-term

GTX102500
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCAPN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calpain 3 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID825
  • Target name
    CAPN3
  • Target description
    calpain 3
  • Target synonyms
    calpain p94, large [catalytic] subunit; calpain, large polypeptide L3; calpain-3; CANP3; CANPL3; LGMD2; LGMD2A; LGMDD4; LGMDR1; muscle-specific calcium-activated neutral protease 3 large subunit; nCL-1; new calpain 1; p94
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20807
  • Protein Name
    Calpain-3
  • Scientific Description
    Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calpain 3/CAPN3 using anti-Calpain 3/CAPN3 antibody (A02170-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 3/CAPN3 antigen affinity purified polyclonal antibody (Catalog # A02170-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 3/CAPN3 at approximately 99 kDa. The expected band size for Calpain 3/CAPN3 is at 99 kDa.
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