![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638748/GTX638748_T-45075_20230616_WB_TPM_watermark_23062019_454.webp)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Calpain 5 antibody [HL2432]
GTX638748
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAPN5
Overview
- SupplierGeneTex
- Product NameCalpain 5 antibody [HL2432]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2432
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID726
- Target nameCAPN5
- Target descriptioncalpain 5
- Target synonymsADNIV, HTRA3, VRNI, nCL-3, calpain-5, calpain htra-3, new calpain 3, testis tissue sperm-binding protein Li 91mP
- HostRabbit
- IsotypeIgG
- Protein IDO15484
- Protein NameCalpain-5
- Scientific DescriptionCalpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638748/GTX638748_T-45075_20230630_WB_M_tissue_23062718_315.webp)
![Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638748/GTX638748_T-45075_20230714_IHC-P_multiple_M_23081619_761.webp)
![Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Calpain 5 stained by Calpain 5 antibody [HL2432] (GTX638748) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638748/GTX638748_T-45075_20230714_IHC-P_R_23081619_362.webp)


![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (GTX103264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103264/GTX103264_44531_20220107_WB_M_uterus_w_23060119_659.webp)
