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Calretinin Rabbit pAb

A9807
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCALB2
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Overview

  • Supplier
    ABclonal
  • Product Name
    Calretinin Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID794
  • Target name
    CALB2
  • Target description
    calbindin 2
  • Target synonyms
    CAB29, CAL2, CR, calretinin, 29 kDa calbindin, calbindin 2, (29kD, calretinin), calbindin D29K, testicular secretory protein Li 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22676
  • Protein Name
    Calretinin
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). Calretinin/CALB2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates probed with Calretinin Polyclonal Antibody, Unconjugated (bs-0062R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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