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CAMK2D / CaMKII Delta Antibody

LS-C331045
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCAMK2D
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CAMK2D / CaMKII Delta Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 54kDa/55kDa/56kDa/57kDa/58kDa/59kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:20 - 1:50), IHC (1:50 - 1:100), WB (1:500 - 1:1000) The predicted MW is 54kDa/55kDa/56kDa/57kDa/58kDa/59kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID817
  • Target name
    CAMK2D
  • Target description
    calcium/calmodulin dependent protein kinase II delta
  • Target synonyms
    calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CAMKD; CaMK-II delta subunit; CaM-kinase II delta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CaMKII delta/CAMK2D using anti-CaMKII delta/CAMK2D antibody (M02611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HUVEC whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII delta/CAMK2D antigen affinity purified monoclonal antibody (M02611) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII delta/CAMK2D at approximately 50 kDa. The expected band size for CaMKII delta/CAMK2D is at 56 kDa.
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