Untreated (–) and treated (+) mouse brain extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Specificity test was performed by coating the plate with CAMK2A peptide, CAMK2A (phospho Thr286) peptide, CAMK2B peptide, CAMK2B (phospho Thr287) peptide, CAMK2G peptide, and CAMK2G (phospho Thr287) peptide (125 ng/mL). Coated peptides were probed with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641517/GTX641517_45439_20250103_ELISA_Indirect_Multiple_Bar_Specificity_25012200_938.webp "Specificity test was performed by coating the plate with CAMK2A peptide, CAMK2A (phospho Thr286) peptide, CAMK2B peptide, CAMK2B (phospho Thr287) peptide, CAMK2G peptide, and CAMK2G (phospho Thr287) peptide (125 ng/mL). Coated peptides were probed with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![CaMKII alpha (phospho Thr286) antibody [HL2908] detects CaMKII alpha (phospho Thr286) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CaMKII alpha (phospho Thr286) stained by CaMKII alpha (phospho Thr286) antibody [HL2908] (GTX641517) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX641517/GTX641517_45439_20250221_ICC_IF_treatment_forskolin_25031220_933.webp "CaMKII alpha (phospho Thr286) antibody [HL2908] detects CaMKII alpha (phospho Thr286) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CaMKII alpha (phospho Thr286) stained by CaMKII alpha (phospho Thr286) antibody [HL2908] (GTX641517) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Untreated (–) and treated (+) mouse brain extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CaMKII alpha (phospho Thr286) antibody [HL3570]
GTX641517
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCAMK2A
Overview
- SupplierGeneTex
- Product NameCaMKII alpha (phospho Thr286) antibody [HL3570]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3570
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID815
- Target nameCAMK2A
- Target descriptioncalcium/calmodulin dependent protein kinase II alpha
- Target synonymsCAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63, calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, caM kinase II subunit alpha, caMK-II subunit alpha, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase type II alpha chain
- HostRabbit
- IsotypeIgG
- Protein IDQ9UQM7
- Protein NameCalcium/calmodulin-dependent protein kinase type II subunit alpha
- Scientific DescriptionThe product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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