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Western blot analysis of extracts of various cell lines using CAPN3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using CAPN3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using CAPN3 Polyclonal Antibody at dilution of 1:3000.

CAPN3 Polyclonal Antibody

E-AB-64116
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CAPN3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calpain 3/CAPN3 using anti-Calpain 3/CAPN3 antibody (A02170-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 3/CAPN3 antigen affinity purified polyclonal antibody (Catalog # A02170-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 3/CAPN3 at approximately 99 kDa. The expected band size for Calpain 3/CAPN3 is at 99 kDa.
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