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ProductsBack/Carbamoyl-phosphate synthase 1 Recombinant Antbody Kit (KD-Validated)

Carbamoyl-phosphate synthase 1 Recombinant Antbody Kit (KD-Validated)

BSM-90036R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCPS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Carbamoyl-phosphate synthase 1 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1373
  • Target name
    CPS1
  • Target description
    carbamoyl-phosphate synthase 1
  • Target synonyms
    carbamoyl-phosphate synthase (ammonia); carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase 1, mitochondrial; carbamoylphosphate synthetase I; CPSASE1; GATD6; PHN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31327
  • Protein Name
    Carbamoyl-phosphate synthase [ammonia], mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPS1 using anti-CPS1 antibody (A01320-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified polyclonal antibody (Catalog # A01320-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
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