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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: CPS1 antibody at 3.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165, 117, 166 kDa Observed band size: 165 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: CPS1 antibody at 3.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165, 117, 166 kDa Observed band size: 165 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: CPS1 antibody at 3.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165, 117, 166 kDa Observed band size: 165 kDa

CPS1 Antibody

CSB-PA005913LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1373
  • Target name
    CPS1
  • Target description
    carbamoyl-phosphate synthase 1
  • Target synonyms
    CPSASE1, GATD6, PHN, carbamoyl-phosphate synthase [ammonia], mitochondrial, carbamoyl-phosphate synthase (ammonia), carbamoyl-phosphate synthase 1, mitochondrial, carbamoylphosphate synthetase I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31327
  • Protein Name
    Carbamoyl-phosphate synthase [ammonia], mitochondrial
  • Scientific Description
    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CPS1 using anti-CPS1 antibody (A01320-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified polyclonal antibody (Catalog # A01320-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
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