Carbonic Anhydrase IX antibody [GT12]
GTX70020
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCA9
Overview
- SupplierGeneTex
- Product NameCarbonic Anhydrase IX antibody [GT12] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityClone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.
- Application Supplier NoteFor ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10microg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT12
- ConjugateUnconjugated
- Gene ID768
- Target nameCA9
- Target descriptioncarbonic anhydrase 9
- Target synonymsCAIX; CA-IX; carbonate dehydratase IX; carbonic anhydrase 9; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; MN; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
- HostMouse
- IsotypeIgG2b
- Protein IDQ16790
- Protein NameCarbonic anhydrase 9
- Scientific DescriptionCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Inflammatory interferon activates HIF-1alpha-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway. Yeh YH et al., 2018 Mar 27, J Exp Clin Cancer ResRead more
- Targeting glioma stem cells through combined BMI1 and EZH2 inhibition. Jin X et al., 2017 Nov, Nat MedRead more
- Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions. Ramchandani D et al., 2016 Dec, Lab InvestRead more
- Bortezomib attenuates HIF-1- but not HIF-2-mediated transcriptional activation. Abd-Aziz N et al., 2015 Oct, Oncol LettRead more
- Expression of hypoxic marker carbonic anhydrase IX predicts poor prognosis in resectable hepatocellular carcinoma. Huang WJ et al., 2015, PLoS OneRead more
- E7080 (lenvatinib), a multi-targeted tyrosine kinase inhibitor, demonstrates antitumor activities against colorectal cancer xenografts. Wiegering A et al., 2014 Nov, NeoplasiaRead more
- Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1alpha by inhibiting prolyl-4-hydroxylase PHD2. Hou P et al., 2014, PLoS OneRead more