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Carbonic Anhydrase IX antibody [GT12]

GTX70020
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCA9
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Overview

  • Supplier
    GeneTex
  • Product Name
    Carbonic Anhydrase IX antibody [GT12] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.
  • Application Supplier Note
    For ICC/IF: Use at a dilution of 1:100-1:1000. For WB: Use at a dilution of 1:100-1:1000. For IHC-P: Use at a dilution of 1:100-1:1000. For FACS: Use at a dilution of 1:100-1:1000 for 1x10^6 cells. For IP: Use at a concentration of 2-10microg/mg lysate. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT12
  • Conjugate
    Unconjugated
  • Gene ID768
  • Target name
    CA9
  • Target description
    carbonic anhydrase 9
  • Target synonyms
    CAIX; CA-IX; carbonate dehydratase IX; carbonic anhydrase 9; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; MN; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ16790
  • Protein Name
    Carbonic anhydrase 9
  • Scientific Description
    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture. Tang X et al., 2021 Sep, Cell Prolif
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  • Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype. Davis RM et al., 2018 Oct 23, ACS Nano
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  • Targeting the Mevalonate Pathway Suppresses VHL-Deficient CC-RCC through an HIF-Dependent Mechanism. Thompson JM et al., 2018 Aug, Mol Cancer Ther
    Read more
  • Inflammatory interferon activates HIF-1alpha-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway. Yeh YH et al., 2018 Mar 27, J Exp Clin Cancer Res
    Read more
  • Targeting glioma stem cells through combined BMI1 and EZH2 inhibition. Jin X et al., 2017 Nov, Nat Med
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  • Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions. Ramchandani D et al., 2016 Dec, Lab Invest
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  • Bortezomib attenuates HIF-1- but not HIF-2-mediated transcriptional activation. Abd-Aziz N et al., 2015 Oct, Oncol Lett
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  • Expression of hypoxic marker carbonic anhydrase IX predicts poor prognosis in resectable hepatocellular carcinoma. Huang WJ et al., 2015, PLoS One
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  • E7080 (lenvatinib), a multi-targeted tyrosine kinase inhibitor, demonstrates antitumor activities against colorectal cancer xenografts. Wiegering A et al., 2014 Nov, Neoplasia
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  • Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1alpha by inhibiting prolyl-4-hydroxylase PHD2. Hou P et al., 2014, PLoS One
    Read more