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Carboxypeptidase A1 / CPA1 (Pancreatic Cancer Marker)(CPA1/2712), CF647 conjugate, 0.1mg/mL [26628-22-8]

Carboxypeptidase A1 / CPA1 (Pancreatic Cancer Marker)(CPA1/2712), CF647 conjugate, 0.1mg/mL [26628-22-8]

BNC472712
Biotium
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCPA1
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Overview

  • Supplier
    Biotium
  • Product Name
    Carboxypeptidase A1 / CPA1 (Pancreatic Cancer Marker)(CPA1/2712), CF647 conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CPA1/2712
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID1357
  • Target name
    CPA1
  • Target description
    carboxypeptidase A1
  • Target synonyms
    carboxypeptidase A1; carboxypeptidase A1 (pancreatic); CPA; pancreatic carboxypeptidase A
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP15085
  • Protein Name
    Carboxypeptidase A1
  • Scientific Description
    Carboxypeptidase A1 (CPA1) is a secreted zinc metalloprotease that is highly expressed in pancreatic tissue. It catalyzes the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Human pancreatic procarboxypeptidase A exists as three different active forms, two of which are carboxypeptidase A1 (CPA1) and carboxypeptidase A2 (CPA2). Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Carboxypeptidase A/CPA1 using anti-Carboxypeptidase A/CPA1 antibody (A05985-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carboxypeptidase A/CPA1 antigen affinity purified polyclonal antibody (Catalog # A05985-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carboxypeptidase A/CPA1 at approximately 47 kDa. The expected band size for Carboxypeptidase A/CPA1 is at 47 kDa.
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