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Western Blot analysis of K562 cells using CPA1 Polyclonal Antibody
Western Blot analysis of K562 cells using CPA1 Polyclonal Antibody
Western Blot analysis of K562 cells using CPA1 Polyclonal Antibody

CPA1 Antibody

CSB-PA001768
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1357
  • Target name
    CPA1
  • Target description
    carboxypeptidase A1
  • Target synonyms
    carboxypeptidase A1; carboxypeptidase A1 (pancreatic); CPA; pancreatic carboxypeptidase A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15085
  • Protein Name
    Carboxypeptidase A1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Carboxypeptidase A/CPA1 using anti-Carboxypeptidase A/CPA1 antibody (A05985-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carboxypeptidase A/CPA1 antigen affinity purified polyclonal antibody (Catalog # A05985-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carboxypeptidase A/CPA1 at approximately 47 kDa. The expected band size for Carboxypeptidase A/CPA1 is at 47 kDa.
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