Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20220812_WB_M_tissue_competitor_watermark_22081423_239.webp "Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20221230_IHC-P_M_22122821_767.webp "Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20221230_IHC-P_R_22122821_467.webp "Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20230331_IHC-P_Cat_23032819_553.webp "Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat heart. Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_T-44669_20220527_WB_heart_w_23061202_912.webp "Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length Human Cardiac Troponin I protein, His tag (GTX109331-pro) (41.65-0.65 nM). Coated protein was probed with Cardiac Troponin I antibody [HL1489] (GTX636963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636963/GTX636963_44732_20240816_ELISA_Indirect_24082301_497.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length Human Cardiac Troponin I protein, His tag (GTX109331-pro) (41.65-0.65 nM). Coated protein was probed with Cardiac Troponin I antibody [HL1489] (GTX636963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cardiac Troponin I antibody [HL1489]
GTX636963
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat
TargetTNNI3
Overview
- SupplierGeneTex
- Product NameCardiac Troponin I antibody [HL1489]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1489
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7137
- Target nameTNNI3
- Target descriptiontroponin I3, cardiac type
- Target synonymsCMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac)
- HostRabbit
- IsotypeIgG
- Protein IDP19429
- Protein NameTroponin I, cardiac muscle
- Scientific DescriptionTroponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
- ReactivityFeline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_40100_IHC_M_w_23060500_480.webp)
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ReactivityHuman, Mouse, Rat
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![Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3-2] (GTX112770) diluted at 1:10000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112770/GTX112770_40079_20160121_WB_R_heart_w_23060500_756.webp)
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