Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500. Scale bar = M μm.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_40100_20200306_IHC-P-FL_M_w_23060500_569.webp "Troponin I (cardiac) antibody detects Troponin I (cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I (cardiac) stained by Troponin I (cardiac) antibody (GTX109331) diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_44349_20220506_WB_M_R_w_23060500_949.webp "Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_40100_IHC_R_w_23060500_672.webp "Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Troponin I (cardiac) antibody [N1C3] (GTX109331) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109331/GTX109331_40100_20200207_WB_M_heart_competitor_watermark_w_23060500_836.webp "Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [N1C3] (GTX109331) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] (GTX109331) dilution: 1:500. Scale bar = M μm.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Cardiac Troponin I antibody [N1C3]
GTX109331
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNNI3
Overview
- SupplierGeneTex
- Product NameCardiac Troponin I antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.05 mg/ml
- ConjugateUnconjugated
- Gene ID7137
- Target nameTNNI3
- Target descriptiontroponin I3, cardiac type
- Target synonymsCMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle, cardiomyopathy, dilated 2A (autosomal recessive), troponin I type 3 (cardiac)
- HostRabbit
- IsotypeIgG
- Protein IDP19429
- Protein NameTroponin I, cardiac muscle
- Scientific DescriptionTroponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Shan SS, Lu SY, Yang YP, et al. A Two-Electrode, Double-Pulsed Sensor Readout Circuit for Cardiac Troponin I Measurement. IEEE Trans Biomed Circuits Syst. 2020,14(6):1362-1370. doi: 10.1109/TBCAS.2020.3029912Read this paper
- Crestani T, Steichen C, Neri E, et al. Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells. Biochem Biophys Res Commun. 2020,533(3):376-382. doi: 10.1016/j.bbrc.2020.09.021Read this paper
Datasheet
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