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ProductsBack/Cardiac Troponin I (TNNI3) Mouse Monoclonal Antibody [Clone ID: 3A10A12]
Western blot analysis using cTnI mouse mAb against truncated cTnI recombinant protein.
Western blot analysis using cTnI mouse mAb against truncated cTnI recombinant protein.
Western blot analysis using cTnI mouse mAb against truncated cTnI recombinant protein.

Cardiac Troponin I (TNNI3) Mouse Monoclonal Antibody [Clone ID: 3A10A12]

AM06110SU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetTNNI3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Cardiac Troponin I (TNNI3) Mouse Monoclonal Antibody [Clone ID: 3A10A12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A10A12
  • Gene ID7137
  • Target name
    TNNI3
  • Target description
    troponin I3, cardiac type
  • Target synonyms
    cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1; troponin I type 3 (cardiac); troponin I, cardiac muscle
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP19429
  • Protein Name
    Troponin I, cardiac muscle
  • Scientific Description
    Cardiac Troponin I (TNNI3) mouse monoclonal antibody, clone 3A10A12, Ascites
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (Catalog # A01720-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 24 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa.
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