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ProductsBack/Cardiac Troponin T (TNNT2) Mouse Monoclonal Antibody [Clone ID: 1G1]
Western blot analysis using Troponin T2 antibody Cat.-No AM06763SU-N against human troponin T2 (AA: 88-249) recombinant protein. (Expected MW is 25.1 kDa)
Western blot analysis using Troponin T2 antibody Cat.-No AM06763SU-N against human troponin T2 (AA: 88-249) recombinant protein. (Expected MW is 25.1 kDa)
Western blot analysis using Troponin T2 antibody Cat.-No AM06763SU-N against human troponin T2 (AA: 88-249) recombinant protein. (Expected MW is 25.1 kDa)

Cardiac Troponin T (TNNT2) Mouse Monoclonal Antibody [Clone ID: 1G1]

AM06763SU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTNNT2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Cardiac Troponin T (TNNT2) Mouse Monoclonal Antibody [Clone ID: 1G1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G1
  • Gene ID7139
  • Target name
    TNNT2
  • Target description
    troponin T2, cardiac type
  • Target synonyms
    CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT, troponin T, cardiac muscle, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, troponin T type 2 (cardiac)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP45379
  • Protein Name
    Troponin T, cardiac muscle
  • Scientific Description
    Cardiac Troponin T (TNNT2) mouse monoclonal antibody, clone 1G1, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
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