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ProductsBack/Casein Kinase 1 alpha (phospho Tyr321) antibody
WB analysis of UV-treated HEK293T (A), Jurkat (B), K562 (C), mouse liver (D), UV-treated H9C2 (E) whole cell lysates using GTX32170 Casein Kinase 1 alpha (phospho Tyr321) antibody.
WB analysis of UV-treated HEK293T (A), Jurkat (B), K562 (C), mouse liver (D), UV-treated H9C2 (E) whole cell lysates using GTX32170 Casein Kinase 1 alpha (phospho Tyr321) antibody.
WB analysis of UV-treated HEK293T (A), Jurkat (B), K562 (C), mouse liver (D), UV-treated H9C2 (E) whole cell lysates using GTX32170 Casein Kinase 1 alpha (phospho Tyr321) antibody.

Casein Kinase 1 alpha (phospho Tyr321) antibody

GTX32170
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCSNK1A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Casein Kinase 1 alpha (phospho Tyr321) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1452
  • Target name
    CSNK1A1
  • Target description
    casein kinase 1 alpha 1
  • Target synonyms
    casein kinase I isoform alpha; CK1; CK1a; CKIa; CKI-alpha; clock regulator kinase; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; HEL-S-77p; HLCDGP1; PRO2975
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48729
  • Protein Name
    Casein kinase I isoform alpha
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Mouse Kidney Tissue Lysate, Lane 4: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK1A1 antigen affinity purified polyclonal antibody (Catalog # PB9693) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK1A1 at approximately 39KD. The expected band size for CSNK1A1 is at 39KD.
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Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detectio
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References
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