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Western Blot analysis of HeLa cells using Casein Kinase Ialpha Polyclonal Antibody
Western Blot analysis of HeLa cells using Casein Kinase Ialpha Polyclonal Antibody
Western Blot analysis of HeLa cells using Casein Kinase Ialpha Polyclonal Antibody

CSNK1A1 Antibody

CSB-PA001224
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCSNK1A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CSNK1A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1452
  • Target name
    CSNK1A1
  • Target description
    casein kinase 1 alpha 1
  • Target synonyms
    casein kinase I isoform alpha; CK1; CK1a; CKIa; CKI-alpha; clock regulator kinase; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; HEL-S-77p; HLCDGP1; PRO2975
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48729
  • Protein Name
    Casein kinase I isoform alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Mouse Kidney Tissue Lysate, Lane 4: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK1A1 antigen affinity purified polyclonal antibody (Catalog # PB9693) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK1A1 at approximately 39KD. The expected band size for CSNK1A1 is at 39KD.
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