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Western Blot analysis of RAW264.7 cells using Caspase-6 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using Caspase-6 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using Caspase-6 Polyclonal Antibody

CASP6 Antibody

CSB-PA001232
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCASP6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CASP6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID839
  • Target name
    CASP6
  • Target description
    caspase 6
  • Target synonyms
    apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; caspase-6; MCH2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55212
  • Protein Name
    Caspase-6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Caspase-6 using anti-Caspase-6 antibody (A02631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6 antigen affinity purified polyclonal antibody (Catalog # A02631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6 at approximately 33KD. The expected band size for Caspase-6 is at 33KD.
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