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ProductsBack/Goat anti-Caspase 6 (alpha)

Goat anti-Caspase 6 (alpha)

EB12165
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCASP6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Caspase 6 (alpha) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID839
  • Target name
    CASP6
  • Target description
    caspase 6
  • Target synonyms
    CSP-6, MCH2, caspase-6, caspase-6, apoptotic protease MCH-2, caspase 6, apoptosis-related cysteine peptidase, caspase 6, apoptosis-related cysteine protease, mammalian Ced-3 homologue 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001217.2; NP_116787.1. GeneIDs all Nonhuman: 12368 (mouse); 83584 (rat);. Purification: Antigen affinity purified. Names and symbols: CASP6; caspase 6, apoptosis-related cysteine peptidase; MCH2; apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine protease; caspase-6
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Caspase-6 using anti-Caspase-6 antibody (A02631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6 antigen affinity purified polyclonal antibody (Catalog # A02631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6 at approximately 33KD. The expected band size for Caspase-6 is at 33KD.
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