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ProductsBack/CASP7 Antibody - N-terminal region (OASG01043)

CASP7 Antibody - N-terminal region (OASG01043)

OASG01043
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCASP7
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CASP7 Antibody - N-terminal region (OASG01043)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID840
  • Target name
    CASP7
  • Target description
    caspase 7
  • Target synonyms
    CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3, caspase-7, ICE-like apoptotic protease 3, apoptotic protease MCH-3, caspase 7, apoptosis-related cysteine peptidase, caspase 7, apoptosis-related cysteine protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-7/CASP7 using anti-Caspase-7/CASP7 antibody (A01044-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-7/CASP7 antigen affinity purified polyclonal antibody (Catalog # A01044-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-7/CASP7 at approximately 35KD. The expected band size for Caspase-7/CASP7 is at 35KD.
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