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WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate
WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate
WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate

Caspase 1 p20 subunit antibody

GTX11701
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCasp1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caspase 1 p20 subunit antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12362
  • Target name
    Casp1
  • Target description
    caspase 1
  • Target synonyms
    ICE, Il1bc, caspase-1, CASP-1, IL-1 beta-converting enzyme, IL-1B converting enzyme, IL-1BC, interleukin-1 beta convertase, interleukin-1 beta-converting enzyme, p45
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29452
  • Protein Name
    Caspase-1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Abd-Ellatif RN, Nasef NA, El-Horany HE, et al. Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis. Int J Mol Sci. 2022,23(23). doi: 10.3390/ijms232314570
    Read this paper
  • Wu YC, Hsu SP, Hu MC, et al. PEP-sNASP Peptide Alleviates LPS-Induced Acute Lung Injury Through the TLR4/TRAF6 Axis. Front Med (Lausanne). 2022,9:832713. doi: 10.3389/fmed.2022.832713
    Read this paper
  • El-Sisi AEE, Sokar SS, Shebl AM, et al. Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibition of TLR4-NF-κB-NLRP3 pathway in hepatic ischemia/reperfusion injury. Toxicol Appl Pharmacol. 2021,410:115340. doi: 10.1016/j.taap.2020.115340
    Read this paper
  • Zhang D, Qian J, Zhang P, et al. Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro. J Neurosci Res. 2019,97(6):645-660. doi: 10.1002/jnr.24385
    Read this paper

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Figure 1. Western blot analysis of CASP1 using anti-CASP1 antibody (PA1440-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 antigen affinity purified polyclonal antibody (Catalog # PA1440-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1 at approximately 46 kDa. The expected band size for CASP1 is at 46 kDa.
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