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WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate
WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate
WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate

Caspase 1 p20 subunit antibody

GTX11701
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCasp1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Caspase 1 p20 subunit antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12362
  • Target name
    Casp1
  • Target description
    caspase 1
  • Target synonyms
    Casp; CASP-1; caspase-1; IC; ICE; Il1; IL-1 beta-converting enzyme; IL-1B converting enzyme; Il1bc; IL-1BC; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; p45
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29452
  • Protein Name
    Caspase-1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis.
    Read more
  • PEP-sNASP Peptide Alleviates LPS-Induced Acute Lung Injury Through the TLR4/TRAF6 Axis. Wu YC et al., 2022, Front Med (Lausanne)
    Read more
  • Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibition of TLR4-NF-kappaB-NLRP3 pathway in hepatic ischemia/reperfusion injury. El-Sisi AEE et al., 2021 Jan 1, Toxicol Appl Pharmacol
    Read more
  • Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro. Zhang D et al., 2019 Jun, J Neurosci Res
    Read more

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Figure 1. Western blot analysis of CASP1 using anti-CASP1 antibody (PA1440-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 antigen affinity purified polyclonal antibody (Catalog # PA1440-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1 at approximately 46 kDa. The expected band size for CASP1 is at 46 kDa.
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