ICC/IF analysis of HeLa cells using Caspase 8 antibody [EPR162] at a dilution of 1:100.
![IHC-P analysis of human hepatocellular carcinoma using Caspase 8 antibody [EPR162] at a dilution of 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX63075/GTX63075_IHC-P_w_23061202_876.webp "IHC-P analysis of human hepatocellular carcinoma using Caspase 8 antibody [EPR162] at a dilution of 1:100.")
![WB analysis of lysates from (1) etoposide-treated Jurkat, (2) untreated Jurkat, (3) staurosporine-treated HeLa and (4) untreated HeLa cells (10 microg per lane) using Caspase 8 antibody [EPR162] at a dilution of 1:1,000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX63075/GTX63075_WB_w_23061202_511.webp "WB analysis of lysates from (1) etoposide-treated Jurkat, (2) untreated Jurkat, (3) staurosporine-treated HeLa and (4) untreated HeLa cells (10 microg per lane) using Caspase 8 antibody [EPR162] at a dilution of 1:1,000.")
ICC/IF analysis of HeLa cells using Caspase 8 antibody [EPR162] at a dilution of 1:100.
Caspase 8 antibody [EPR162]
GTX63075
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCASP8
Overview
- SupplierGeneTex
- Product NameCaspase 8 antibody [EPR162]
- Delivery Days Customer9
- Application Supplier NoteRecommended Starting Dilutions: For WB: Use at a dilution of 1:1000 - 1:10000. For IHC-P: Use at a dilution of 1:100 - 1:250. For ICC/IF: Use at a dilution of 1:100 - 1:250. Optimal dilutions should be determined experimentally by the researcher.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEPR162
- ConjugateUnconjugated
- Gene ID841
- Target nameCASP8
- Target descriptioncaspase 8
- Target synonymsALPS2B; apoptotic cysteine protease; apoptotic protease Mch-5; CAP4; Casp-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MORT1-associated ced-3 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ14790
- Protein NameCaspase-8
- Scientific DescriptionThis gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203