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Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with caspase-8 subunit p18 Polyclonal Antibody, Unconjugated (bs-6463R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with caspase-8 subunit p18 Polyclonal Antibody, Unconjugated (bs-6463R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with caspase-8 subunit p18 Polyclonal Antibody, Unconjugated (bs-6463R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Caspase-8 subunit p18 Antibody

BS-6463R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCASP8
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Overview

  • Supplier
    Bioss
  • Product Name
    Caspase-8 subunit p18 Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID841
  • Target name
    CASP8
  • Target description
    caspase 8
  • Target synonyms
    ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, caspase-8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein, MORT1-associated ced-3 homolog, apoptotic cysteine protease, apoptotic protease Mch-5, caspase 8, apoptosis-related cysteine peptidase, caspase 8, apoptosis-related cysteine protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14790
  • Protein Name
    Caspase-8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • You XG, Fan TJ, Jiang GJ. Phenylephrine induces necroptosis and apoptosis in corneal epithelial cells dose- and time-dependently. Toxicology. 2019,428:152305. doi: 10.1016/j.tox.2019.152305
    Read this paper
  • Bozok Cetintas V, Acikgoz E, Yigitturk G, et al. Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells. Medicine (Baltimore). 2016,95(43):e5150. doi: 10.1097/MD.0000000000005150
    Read this paper
  • Zhao J, Qiu Y, Tian CL, et al. The cytotoxic and pro-apoptotic effects of phenylephrine on corneal stromal cells via a mitochondrion-dependent pathway both in vitro and in vivo. Exp Toxicol Pathol. 2016,68(7):409-17. doi: 10.1016/j.etp.2016.06.003
    Read this paper
  • Shan M, Fan TJ. Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway. Toxicol In Vitro. 2016,35:36-42. doi: 10.1016/j.tiv.2016.05.008
    Read this paper