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CASR Antibody (OASG01067)

OASG01067
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCASR
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CASR Antibody (OASG01067)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Gene ID846
  • Target name
    CASR
  • Target description
    calcium sensing receptor
  • Target synonyms
    CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR, extracellular calcium-sensing receptor, parathyroid Ca(2+)-sensing receptor 1, parathyroid cell calcium-sensing receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CASR using anti-CASR antibody (PB9924). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASR antigen affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASR at approximately 150 kDa. The expected band size for CASR is at 121 kDa.
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