
Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20microg). Lane 2: NBLS Nuclear (3microg). Lane 3: BE2C Cytoplasmic (30microg). Lane 4: BE2C Nuclear (7microg). Lane 5: SY5Y-CASZ1b (10microg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.
Casz1 antibody
GTX48704
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetCASZ1
Overview
- SupplierGeneTex
- Product NameCasz1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:5000. ELISA: 1:45000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.07 mg/ml
- ConjugateUnconjugated
- Gene ID54897
- Target nameCASZ1
- Target descriptioncastor zinc finger 1
- Target synonymsCAS11; castor homolog 1, zinc finger; castor-related protein; CST; dJ734G22.1; SRG; survival-related; zinc finger protein 693; zinc finger protein castor homolog 1; ZNF693
- HostRabbit
- IsotypeIgG
- Protein IDQ86V15
- Protein NameZinc finger protein castor homolog 1
- Scientific DescriptionThe human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203