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Western Blot analysis of HepG2 cells using Caveolin-1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Caveolin-1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Caveolin-1 Polyclonal Antibody

CAV1 Antibody

CSB-PA001332
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAV1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CAV1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID857
  • Target name
    CAV1
  • Target description
    caveolin 1
  • Target synonyms
    BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21, caveolin-1, caveolin 1, caveolae protein, 22kDa, cell growth-inhibiting protein 32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03135
  • Protein Name
    Caveolin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: rat ovary tissue lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse ovary tissue lysates, Lane 9: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-1/CAV1 antigen affinity purified polyclonal antibody (Catalog # PB9165) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.
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