Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Caveolin 1 antibody [N1N3] detects Caveolin 1 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Caveolin 1 protein stained by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. Blue: Hoechst 33343 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100205/GTX100205_39274_IFA_w_23053123_803.webp "Caveolin 1 antibody [N1N3] detects Caveolin 1 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Caveolin 1 protein stained by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemical microscopy analysis of paraffin-embedded lung carcinoma cell line xenograft tissue using Caveolin-1 antibody [N1N3] (GTX100205) (1:100).
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100205/GTX100205_IHC_w_23053123_317.webp "Immunohistochemical microscopy analysis of paraffin-embedded lung carcinoma cell line xenograft tissue using Caveolin-1 antibody [N1N3] (GTX100205) (1:100).
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Caveolin 1 antibody [N1N3] detects Caveolin 1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100205/GTX100205_39274_20151029_WB_M_lung_w_23053123_423.webp "Caveolin 1 antibody [N1N3] detects Caveolin 1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of Caveolin 1 protein from A549 membrane extracts using 5 μg of Caveolin 1 antibody [N1N3] (GTX100205). Western blot analysis was performed using Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100205/GTX100205_39274_20150106_IP_w_23060100_899.webp "Immunoprecipitation of Caveolin 1 protein from A549 membrane extracts using 5 μg of Caveolin 1 antibody [N1N3] (GTX100205). Western blot analysis was performed using Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Caveolin 1 antibody [N1N3]
GTX100205
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAV1
Overview
- SupplierGeneTex
- Product NameCaveolin 1 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID857
- Target nameCAV1
- Target descriptioncaveolin 1
- Target synonymsBSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21, caveolin-1, caveolin 1, caveolae protein, 22kDa, cell growth-inhibiting protein 32
- HostRabbit
- IsotypeIgG
- Protein IDQ03135
- Protein NameCaveolin-1
- Scientific DescriptionThe scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cinato M, Guitou L, Saidi A, et al. Apilimod alters TGFβ signaling pathway and prevents cardiac fibrotic remodeling. Theranostics. 2021,11(13):6491-6506. doi: 10.7150/thno.55821Read this paper
- Cooper-Knock J, Zhang S, Kenna KP, et al. Rare Variant Burden Analysis within Enhancers Identifies CAV1 as an ALS Risk Gene. Cell Rep. 2020,33(9):108456. doi: 10.1016/j.celrep.2020.108456Read this paper
- Fang CH, Lin YT, Liang CM, et al. A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling. J Biomed Sci. 2020,27(1):42. doi: 10.1186/s12929-020-00638-xRead this paper
- Dong X, Song Q, Zhu J, et al. Interaction of Caveolin-3 and HCN is involved in the pathogenesis of diabetic cystopathy. Sci Rep. 2016,6:24844. doi: 10.1038/srep24844Read this paper
- Hayashi T, Ichimura T, Yaegashi N, et al. Expression of CAVEOLIN 1 in uterine mesenchymal tumors: No relationship between malignancy and CAVEOLIN 1 expression. Biochem Biophys Res Commun. 2015,463(4):982-7. doi: 10.1016/j.bbrc.2015.06.046Read this paper
Datasheet
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