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ProductsBack/CAV2 / Caveolin 2 Antibody

CAV2 / Caveolin 2 Antibody

LS-C760883
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCAV2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CAV2 / Caveolin 2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID858
  • Target name
    CAV2
  • Target description
    caveolin 2
  • Target synonyms
    CAV, caveolin-2, caveolae protein, 20-kD
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caveolin-2/CAV2 using anti-Caveolin-2/CAV2 antibody (A01574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: mouse skeletal muscle tissue lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-2/CAV2 antigen affinity purified polyclonal antibody (Catalog # A01574) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-2/CAV2 at approximately 22KD. The expected band size for Caveolin-2/CAV2 is at 22KD.
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