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Caveolin-3 Recombinant Antibody, Biotin Conjugated

BSM-61350R-BIOTIN
Bioss
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAV3
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Overview

  • Supplier
    Bioss
  • Product Name
    Caveolin-3 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB(1:300-5000), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID859
  • Target name
    CAV3
  • Target description
    caveolin 3
  • Target synonyms
    LGMD1C, LQT9, MPDT, RMD2, VIP-21, VIP21, caveolin-3, M-caveolin, cavolin 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56539
  • Protein Name
    Caveolin-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CAV3 using anti-CAV3 antibody (A00990-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 antigen affinity purified polyclonal antibody (Catalog # A00990-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV3 at approximately 22KD. The expected band size for CAV3 is at 17KD.
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