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Western Blot analysis of 293 cells using Cbl Polyclonal Antibody
Western Blot analysis of 293 cells using Cbl Polyclonal Antibody
Western Blot analysis of 293 cells using Cbl Polyclonal Antibody

CBL Antibody

CSB-PA001340
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCBL
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Overview

  • Supplier
    Cusabio
  • Product Name
    CBL Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID867
  • Target name
    CBL
  • Target description
    Cbl proto-oncogene
  • Target synonyms
    C-CBL, CBL2, FRA11B, NSLL, RNF55, E3 ubiquitin-protein ligase CBL, Cas-Br-M (murine) ecotropic retroviral transforming sequence, Cbl proto-oncogene, E3 ubiquitin protein ligase, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, casitas B-lineage lymphoma proto-oncogene, fragile site, folic acid type, rare, fra(11)(q23.3), oncogene CBL2, proto-oncogene c-Cbl, signal transduction protein CBL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22681
  • Protein Name
    E3 ubiquitin-protein ligase CBL
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CBL using anti-CBL antibody (A00152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human CCRF-CEM whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human Hela whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # A00152-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBL at approximately 120 kDa. The expected band size for CBL is at 120 kDa.
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