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ProductsBack/CBR / CBR1 Antibody

CBR / CBR1 Antibody

LS-C812903
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCBR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CBR / CBR1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID873
  • Target name
    CBR1
  • Target description
    carbonyl reductase 1
  • Target synonyms
    CBR, PG-9-KR, SDR21C1, hCBR1, carbonyl reductase [NADPH] 1, 15-hydroxyprostaglandin dehydrogenase, 20-beta-hydroxysteroid dehydrogenase, NADPH-dependent carbonyl reductase 1, alcohol dehydrogenase [NAD(P)+] CBR1, carbonyl reductase (NADPH) 1, epididymis secretory sperm binding protein, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, short chain dehydrogenase/reductase family 21C member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody (A02825-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified polyclonal antibody (Catalog # A02825-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 36 kDa. The expected band size for CBR1 is at 30 kDa.
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