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Goat anti-CBR1

EB05515
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCBR1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CBR1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID873
  • Target name
    CBR1
  • Target description
    carbonyl reductase 1
  • Target synonyms
    CBR, PG-9-KR, SDR21C1, hCBR1, carbonyl reductase [NADPH] 1, 15-hydroxyprostaglandin dehydrogenase, 20-beta-hydroxysteroid dehydrogenase, NADPH-dependent carbonyl reductase 1, alcohol dehydrogenase [NAD(P)+] CBR1, carbonyl reductase (NADPH) 1, epididymis secretory sperm binding protein, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, short chain dehydrogenase/reductase family 21C member 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001748.1. Purification: Antigen affinity purified. Names and symbols: CBR1; carbonyl reductase 1; CBR; carbonyl reductase (NADPH); carbonyl reductase (NADPH) 1; hCBR1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; SDR21C1; short chain dehydrogenase/reductase family 21C, member 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody (A02825-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified polyclonal antibody (Catalog # A02825-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 36 kDa. The expected band size for CBR1 is at 30 kDa.
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