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Western Blot Positive WB detected in:HEK293 whole cell lysate,PC-3 whole cell lysate,Mouse kidney tissue,Mouse brain tissue All lanes:CBR1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31,19 KDa Observed band size: 31 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate,PC-3 whole cell lysate,Mouse kidney tissue,Mouse brain tissue All lanes:CBR1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31,19 KDa Observed band size: 31 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate,PC-3 whole cell lysate,Mouse kidney tissue,Mouse brain tissue All lanes:CBR1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31,19 KDa Observed band size: 31 KDa

CBR1 Antibody

CSB-PA004586LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCBR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CBR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID873
  • Target name
    CBR1
  • Target description
    carbonyl reductase 1
  • Target synonyms
    CBR, PG-9-KR, SDR21C1, hCBR1, carbonyl reductase [NADPH] 1, 15-hydroxyprostaglandin dehydrogenase, 20-beta-hydroxysteroid dehydrogenase, NADPH-dependent carbonyl reductase 1, alcohol dehydrogenase [NAD(P)+] CBR1, carbonyl reductase (NADPH) 1, epididymis secretory sperm binding protein, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, short chain dehydrogenase/reductase family 21C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16152
  • Protein Name
    Carbonyl reductase [NADPH] 1
  • Scientific Description
    NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody (A02825-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified polyclonal antibody (Catalog # A02825-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 36 kDa. The expected band size for CBR1 is at 30 kDa.
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