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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue All lanes: CBS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 62 kDa Observed band size: 61 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue All lanes: CBS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 62 kDa Observed band size: 61 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Mouse liver tissue All lanes: CBS antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 62 kDa Observed band size: 61 kDa

CBS Antibody

CSB-PA12719A0RB
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCBS
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Overview

  • Supplier
    Cusabio
  • Product Name
    CBS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID875
  • Target name
    CBS
  • Target description
    cystathionine beta-synthase
  • Target synonyms
    CBSL, HIP4, cystathionine beta-synthase, Cystathionine beta-synthase-like protein, beta-thionase, methylcysteine synthase, serine sulfhydrase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35520
  • Protein Name
    Cystathionine beta-synthase
  • Scientific Description
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CBS using anti-CBS antibody (A00130-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: huamn HEK293 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (Catalog # A00130-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBS at approximately 63 kDa. The expected band size for CBS is at 63 kDa.
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