CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS stained by CBS antibody (GTX113400) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 μg CBS antibody (GTX113400) 7.5 % SDS-PAGE The immunoprecipitated CBS protein was detected by CBS antibody (GTX113400) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX113400/GTX113400_40128_IP_w_23060501_909.webp "CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract A. 50 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of CBS protein by 4 μg CBS antibody (GTX113400) 7.5 % SDS-PAGE The immunoprecipitated CBS protein was detected by CBS antibody (GTX113400) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CBS stained by CBS antibody (GTX113400) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113400/GTX113400_44587_20220422_ICC_IF_w_23060501_376.webp "CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CBS stained by CBS antibody (GTX113400) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS stained by CBS antibody (GTX113400) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CBS antibody
GTX113400
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCBS
Overview
- SupplierGeneTex
- Product NameCBS antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.81 mg/ml
- ConjugateUnconjugated
- Gene ID875
- Target nameCBS
- Target descriptioncystathionine beta-synthase
- Target synonymsCBSL, HIP4, cystathionine beta-synthase, Cystathionine beta-synthase-like protein, beta-thionase, methylcysteine synthase, serine sulfhydrase
- HostRabbit
- IsotypeIgG
- Protein IDP0DN79
- Scientific DescriptionThe protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wijerathne CUB, Madduma Hewage S, Siow YL, et al. Kidney Ischemia-Reperfusion Decreases Hydrogen Sulfide and Increases Oxidative Stress in the Heart. Biomolecules. 2020,10(11). doi: 10.3390/biom10111565Read this paper
- Morales-Loredo H, Barrera A, Garcia JM, et al. Hydrogen sulfide regulation of renal and mesenteric blood flow. Am J Physiol Heart Circ Physiol. 2019,317(5):H1157-H1165. doi: 10.1152/ajpheart.00303.2019Read this paper
Datasheet
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