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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA557525(CBX8 Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA557525(CBX8 Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA557525(CBX8 Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: x200)

CBX8 Antibody

CSB-PA557525
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCBX8
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Overview

  • Supplier
    Cusabio
  • Product Name
    CBX8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57332
  • Target name
    CBX8
  • Target description
    chromobox 8
  • Target synonyms
    PC3, RC1, chromobox protein homolog 8, Pc class 3 homolog, chromobox homolog 8 (Pc class homolog, Drosophila), polycomb 3, rectachrome 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC52
  • Protein Name
    Chromobox protein homolog 8
  • Scientific Description
    Chromobox protein homolog 8 is a protein that in humans is encoded by the CBX8 gene.CBX8 has been shown to interact with RING1 and MLLT1.Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119, rendering chromatin heritably changed in its expressibility.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cbx8 using anti-Cbx8 antibody (A05234-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Colo320 whole cell lysates, Lane 5: huamn Raji whole cell lysates, Lane 6: huamn SW620 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cbx8 antigen affinity purified polyclonal antibody (Catalog # A05234-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cbx8 at approximately 43-50KD. The expected band size for Cbx8 is at 43KD.
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