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CBX8 Antibody

LS-C334571
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCBX8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CBX8 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 43kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:10 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 43kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57332
  • Target name
    CBX8
  • Target description
    chromobox 8
  • Target synonyms
    PC3, RC1, chromobox protein homolog 8, Pc class 3 homolog, chromobox homolog 8 (Pc class homolog, Drosophila), polycomb 3, rectachrome 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cbx8 using anti-Cbx8 antibody (A05234-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Colo320 whole cell lysates, Lane 5: huamn Raji whole cell lysates, Lane 6: huamn SW620 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cbx8 antigen affinity purified polyclonal antibody (Catalog # A05234-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cbx8 at approximately 43-50KD. The expected band size for Cbx8 is at 43KD.
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