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ProductsBack/CCDC36 Antibody (aa422-450)

CCDC36 Antibody (aa422-450)

LS-C168415
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIHO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCDC36 Antibody (aa422-450)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:10 - 1:50), IHC, IHC-P (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID339834
  • Target name
    IHO1
  • Target description
    interactor of HORMAD1 1
  • Target synonyms
    cancer/testis antigen 74; CCDC36; coiled-coil domain containing 36; coiled-coil domain-containing protein 36; CT74; interactor of HORMAD1 protein 1; LELA1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCDC36 using anti-CCDC36 antibody (A14775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC36 antigen affinity purified polyclonal antibody (Catalog # A14775) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC36 at approximately 66KD. The expected band size for CCDC36 is at 66KD.
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