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Western Blot analysis of HepG2 cell using CCL16 Polyclonal Antibody at dilution of 1:760
Western Blot analysis of HepG2 cell using CCL16 Polyclonal Antibody at dilution of 1:760
Western Blot analysis of HepG2 cell using CCL16 Polyclonal Antibody at dilution of 1:760

CCL16 Polyclonal Antibody

E-AB-16304
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CCL16 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL16 using anti-CCL16 antibody (A07545-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL16 antigen affinity purified polyclonal antibody (Catalog # A07545-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL16 at approximately 18KD. The expected band size for CCL16 is at 14KD.
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