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Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCL20 Polyclonal Antibody, Unconjugated (bs-1268R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCL20 Polyclonal Antibody, Unconjugated (bs-1268R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CCL20 Polyclonal Antibody, Unconjugated (bs-1268R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

CCL20 Polyclonal Antibody

BS-1268R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCcl20
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Overview

  • Supplier
    Bioss
  • Product Name
    CCL20 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID20297
  • Target name
    Ccl20
  • Target description
    C-C motif chemokine ligand 20
  • Target synonyms
    CKb4, LARC, MIP-3A, MIP-3[a], MIP3A, ST38, Scya20, exodus-1, C-C motif chemokine 20, CC chemokine LARC, CC chemokine ST38, MIP-3-alpha, beta-chemokine exodus-1, chemokine (C-C motif) ligand 20, liver and activation-regulated chemokine, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A20, small-inducible cytokine A20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO89093
  • Protein Name
    C-C motif chemokine 20
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu Y, Yu HJ, Wang N, et al. Clara cell 10-kDa protein inhibits T(H)17 responses through modulating dendritic cells in the setting of allergic rhinitis. J Allergy Clin Immunol. 2013,131(2):387-94.e1-12. doi: 10.1016/j.jaci.2012.11.027
    Read this paper

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Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.
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