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Macrophage Inflammatory Protein 3 Alpha (MIP3a) Polyclonal Antibody

CAU28632
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCcl20
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Overview

  • Supplier
    Biomatik
  • Product Name
    Macrophage Inflammatory Protein 3 Alpha (MIP3a) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID20297
  • Target name
    Ccl20
  • Target description
    C-C motif chemokine ligand 20
  • Target synonyms
    CKb4, LARC, MIP-3A, MIP-3[a], MIP3A, ST38, Scya20, exodus-1, C-C motif chemokine 20, CC chemokine LARC, CC chemokine ST38, MIP-3-alpha, beta-chemokine exodus-1, chemokine (C-C motif) ligand 20, liver and activation-regulated chemokine, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A20, small-inducible cytokine A20
  • Host
    Rabbit
  • Protein IDO89093
  • Protein Name
    C-C motif chemokine 20
  • Scientific Description
    The Macrophage Inflammatory Protein 3 Alpha (MIP3a) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.
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ReactivityMouse, Rat
TargetCcl20
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