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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated (bs-1324R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated (bs-1324R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated (bs-1324R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

RANTES Polyclonal Antibody

BS-1324R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCL5
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Overview

  • Supplier
    Bioss
  • Product Name
    RANTES Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6352
  • Target name
    CCL5
  • Target description
    C-C motif chemokine ligand 5
  • Target synonyms
    D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP, C-C motif chemokine 5, T-cell specific protein p288, beta-chemokine RANTES, chemokine (C-C motif) ligand 5, eosinophil chemotactic cytokine, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine subfamily A (Cys-Cys), member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13501
  • Protein Name
    C-C motif chemokine 5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Irie K, Tomofuji T, Ekuni D, et al. Age-related changes of CD4(+) T cell migration and cytokine expression in germ-free and SPF mice periodontium. Arch Oral Biol. 2018,87:72-78. doi: 10.1016/j.archoralbio.2017.12.007
    Read this paper
  • Sun Y, Roberts A, Mauerhan DR, et al. Biological effects and osteoarthritic disease-modifying activity of small molecule CM-01. J Orthop Res. 2018,36(1):309-317. doi: 10.1002/jor.23616
    Read this paper
  • Sun Y, Haines N, Roberts A, et al. Disease-modifying effects of phosphocitrate and phosphocitrate-β-ethyl ester on partial meniscectomy-induced osteoarthritis. BMC Musculoskelet Disord. 2015,16:270. doi: 10.1186/s12891-015-0724-x
    Read this paper

Datasheet

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Figure 1. Western blot analysis of RANTES using anti-RANTES antibody (PB9573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HUT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANTES antigen affinity purified polyclonal antibody (Catalog # PB9573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANTES at approximately 11 kDa. The expected band size for RANTES is at 11 kDa.
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