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ProductsBack/RANTES Antibody (YA2383, Rabbit)

RANTES Antibody (YA2383, Rabbit)

HY-P82638
MedChem Express
TargetCCL5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RANTES Antibody (YA2383, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6352
  • Target name
    CCL5
  • Target description
    C-C motif chemokine ligand 5
  • Target synonyms
    D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP, C-C motif chemokine 5, T-cell specific protein p288, beta-chemokine RANTES, chemokine (C-C motif) ligand 5, eosinophil chemotactic cytokine, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine subfamily A (Cys-Cys), member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RANTES Antibody (YA2383) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2383), targeting RANTES, with a predicted molecular weight of 10 kDa (observed band size: 10 kDa). RANTES Antibody (YA2383) can be used for WB, ICC/IF experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RANTES using anti-RANTES antibody (PB9573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HUT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANTES antigen affinity purified polyclonal antibody (Catalog # PB9573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANTES at approximately 11 kDa. The expected band size for RANTES is at 11 kDa.
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