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Western Blot analysis of HeLa cells using Cyclin A1 Polyclonal Antibody
Western Blot analysis of HeLa cells using Cyclin A1 Polyclonal Antibody
Western Blot analysis of HeLa cells using Cyclin A1 Polyclonal Antibody

CCNA1 Antibody

CSB-PA001859
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCNA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCNA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8900
  • Target name
    CCNA1
  • Target description
    cyclin A1
  • Target synonyms
    CT146, cyclin-A1, testicular tissue protein Li 34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78396
  • Protein Name
    Cyclin-A1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (PB9485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/CCNA1 antigen affinity purified polyclonal antibody (Catalog # PB9485) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/CCNA1 at approximately 55 kDa. The expected band size for Cyclin A1/CCNA1 is at 52 kDa.
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