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Formalin-fixed and paraffin embedded rat ovary labeled with Rabbit Anti-Cyclin A1 Polyclonal Antibody, Unconjugated (bs-5739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat ovary labeled with Rabbit Anti-Cyclin A1 Polyclonal Antibody, Unconjugated (bs-5739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat ovary labeled with Rabbit Anti-Cyclin A1 Polyclonal Antibody, Unconjugated (bs-5739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n

Cyclin A1 Polyclonal Antibody

BS-5739R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCNA1
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclin A1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8900
  • Target name
    CCNA1
  • Target description
    cyclin A1
  • Target synonyms
    CT146, cyclin-A1, testicular tissue protein Li 34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78396
  • Protein Name
    Cyclin-A1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang L, Wei J, Duan J, et al. Silica nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater. 2020,384:121361. doi: 10.1016/j.jhazmat.2019.121361
    Read this paper
  • Zhao Y, Feng Y, Zhang H, et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Theriogenology. 2017,89:280-288. doi: 10.1016/j.theriogenology.2016.11.014
    Read this paper
  • Ghate NB, Das A, Chaudhuri D, et al. Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio. Cell Death Discov. 2016,2:15062. doi: 10.1038/cddiscovery.2015.62
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (PB9485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/CCNA1 antigen affinity purified polyclonal antibody (Catalog # PB9485) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/CCNA1 at approximately 55 kDa. The expected band size for Cyclin A1/CCNA1 is at 52 kDa.
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